Novel Fluorescent Glycan Microarray Strategy Reveals Ligands for Galectins
نویسندگان
چکیده
منابع مشابه
Novel fluorescent glycan microarray strategy reveals ligands for galectins.
Galectin-1 (Gal-1) and galectin-3 (Gal-3) are widely expressed galectins with immunoregulatory functions in animals. To explore their glycan specificity, we developed microarrays of naturally occurring glycans using a bifunctional fluorescent linker, 2-amino-N-(2-aminoethyl)-benzamide (AEAB), directly conjugated through its arylamine group by reductive amination to free glycans to form glycan-A...
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Glycans, which attach to some lipids and to Asn/Ser/Thr residues of proteins, draw attention as the third biological chains next to DNA and protein, since they play a key role in embryogenesis, immunity and diseases. Glycans consist of carbohydrate sugars and their derivatives such as glucose (Glc), mannose (Man), N-acetyl-glucosamine (GlcNAc) and sialic acid (Neu5Ac), and form linear and branc...
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ژورنال
عنوان ژورنال: Chemistry & Biology
سال: 2009
ISSN: 1074-5521
DOI: 10.1016/j.chembiol.2008.11.004